replacement vector meaning in Chinese

取代载体
置换载体

To clone large and random dna fragments , the second generation of yac , pjs97 - pjs98 was modified as gene replacement vector in streptomyces
4kb和1 . okb片段，而且还插入了勿，七刃j和spc心t厂抗性基因。
Phz621 - phz622 , the new gene replacement vector system , had been constructed through the insertion of the 1 . 0kb and 1 . 4kb flanking sequences of hau3r gene from wild - type s . lividans in the same natural orientation
预期在携带有大插入片段的基因置换yac分子进入野生型变铅青链霉菌后， yac分子将通过1 . 4kb或1
In this study , we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s . avermitilis olm73 - 12 , producing only avermectins b and no oligomycin . gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5 . avermitilis olm73 - 12 protoplasts
我们以不产寡霉素而仅产阿维菌素b的工程菌olm73 - 12为出发菌株，用委内瑞拉链霉菌( streptomycesvenezuelae )中编码pikromycinpks模块2上完全活性的dh和酮基还原酶( kr )的dna区域对olm73 - 12染色体上编码阿维菌素pks模块2中dh和kr的区域进行取代，试图构建仅产b1组分的基因工程菌。